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Patients with persistent heartburn despite optimal PPI therapy or those with alarm symptoms (difficulty swallowing, weight loss, anemia) should be referred for endoscopic evaluation according to clinical guidelines

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Fluorescence before and after robotic addition of GLP-1 was serially read using a Flexstation 3 with 485 nm excitation, 525 nm emission (cut-off 515 nm), and expressed relative to average baseline to establish fold change, with GLP-1 concentration response data analyzed by four-parameter fit

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